Journal: Oncology Reports

Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation

doi: 10.3892/or.2026.9086

Figure Lengend Snippet: Knockdown of CCT2 inhibits STAT3 signaling activation in hepatocellular carcinoma cells. The protein levels of STAT3, p-STAT3, MCL1, MMP2 and SOX2 in (A) Huh-7 and (B) HCCLM3 cells were measured by western blotting. The protein levels of (C) p-STAT3, (D) MCL1, (E) MMP2 and (F) SOX2 in subcutaneous tumor tissue were detected by immunohistochemical staining. *P<0.05, **P<0.01 vs. sh-NC. CCT2, chaperonin containing TCP1 subunit 2; sh, short hairpin; NC, negative control; p, phosphorylated; MCL1, myeloid cell leukemia sequence 1; SOX2, SRY-box transcription factor 2.

Article Snippet: The primary antibodies were as follows: CCT2 (cat. no. 24896-1-AP), β-actin (cat. no. 66009-1-Ig), MMP2 (cat. no. 10373-2-AP), myeloid cell leukemia sequence 1 (MCL1; cat. no. 16225-1-AP) and SRY-box transcription factor 2 (SOX2; cat. no. 11064-1-AP; all Proteintech Group, Inc.) and STAT3 (cat. no. 4904) and phosphorylated (p-)STAT3 (Tyr705; cat. no. 4113; both Cell Signaling Technology, Inc.) The membranes were washed three times in TBST (0.1% Tween-20) for 5 min each at room temperature.

Techniques: Knockdown, Activation Assay, Western Blot, Immunohistochemical staining, Staining, Negative Control, Sequencing

Journal: Scientific Reports

Article Title: A lncRNA and radiomics-based model for predicting the response of non-small cell lung cancer to chemo- and radio-therapy

doi: 10.1038/s41598-026-39560-x

Figure Lengend Snippet: ( A ) GO enrichment analysis of 1650 targeted mRNAs. ( B ) KEGG pathway enrichment analysis of 1650 targeted mRNAs . ( C ) The ceRNA network map of MIF-AS1. ( D ) Analysis of the correlation between MIF-AS1 expression and RAD21 expression in NSCLC tumors from the TCGA database (Pearson correlation analysis). ( E ) Knockdown of MIF-AS1 by siRNA decreases RAD21 expression in NSCLC PC9 cells. PC9 cells were transfected with control (si-NC) and MIF-AS1 siRNAs (si-MIF-AS1-1), and the mRNA and protein levels of RAD21 were determined by qPCR and western blot (Student’s t test). ( F ) KM survival analysis of RAD21 in the TCGA dataset. NSCLC patients were divided into low-expression and high-expression groups based on the optimal cut-off point.

Article Snippet: The membranes were blocked with 5% non-fat milk in TBST for 2 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies: anti-Rad21 rabbit monoclonal antibody (Absin, Shanghai, China; 1:1000) and anti-β-Actin mouse monoclonal antibody (Proteintech, Wuhan, China; 1:5000).

Techniques: Expressing, Knockdown, Transfection, Control, Western Blot

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb attenuates OA progression. A . Representative images of H&E staining of synovium and cartilage, SO-FG and TB staining of mouse knee joints. Scale bar = 100 μm. n = 6. B . Synovitis score comparing the severity of synovial inflammation in mice. n = 6. C . OARSI scoring system comparing cartilage degradation in mice. n = 6. D . Representative IHC images of COL2A and MMP13 in mouse knee cartilage. Scale bar = 100 μm. n = 6. E . Quantitative analysis of COL2A-positive area in cartilage. n = 6. F . Quantitative analysis of MMP13-positive area in cartilage. n = 6. G . Representative micro-CT 3D reconstruction images of mouse knee joints and osteophytes (indicated by red arrows). n = 6. H . Quantitative analysis of osteophyte numbers. n = 6. I . Representative coronal micro-CT images of mouse knee joints. n = 6. J . Quantitative analysis of subchondral bone volume fraction (BV/TV) and trabecular thickness (Tb.Th). n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: Staining, Micro-CT

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb regulates chondrocyte function via synovial macrophages. A . Schematic diagram of the macrophage–chondrocyte co-culture system. B . Western blot analysis of COL2A, Aggrecan, MMP13, and ADAMTS5 protein levels in chondrocytes under different co-culture conditions, and the quantitative analysis of the protein. n = 3. C . Representative micromass Alcian blue staining of chondrocytes under different co-culture conditions. n = 3. D . Flow cytometry analysis of chondrocyte proliferation under different co-culture conditions, with quantitative analysis. n = 6. E . Representative EdU staining images and quantitative analysis of chondrocyte proliferation under different co-culture conditions. Scale bar = 100 μm. n = 6. F . Flow cytometry analysis of chondrocyte apoptosis under different co-culture conditions, with quantitative analysis. n = 6. G . TUNEL assay for chondrocyte apoptosis under different co-culture conditions, with quantitative analysis. Scale bar = 100 μm. n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: Co-Culture Assay, Western Blot, Staining, Flow Cytometry, TUNEL Assay

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: Gb suppresses NLRP3 inflammasome-mediated macrophage inflammation and protects chondrocytes. A . Heatmap of RNA-seq analysis comparing Gb-treated and control macrophages. B . GSEA indicating significant reduction in cytokine activity and metalloproteinase activity in Gb-treated macrophages. C . GO enrichment analysis showing differential mRNAs are mainly involved in maintaining cell polarization, regulating inflammatory response, and regulating NLRP3 signaling. D . Western blot analysis of NLRP3 knockdown efficiency in macrophages. n = 3. E . IF analysis of NLRP3 knockdown efficiency. Scale bar = 100 μm. n = 6. F . Western blot analysis of Caspase-1 and IL-1β expression in shNLRP3 macrophages treated with Gb. n = 3. G . qRT-PCR analysis of IL-1β, TNF-α, and IL-10 mRNA in shNLRP3 macrophages treated with Gb. n = 3. H . Representative micromass Alcian blue staining of chondrocytes under different co-culture conditions. n = 3. I . Western blot analysis of COL2A, Aggrecan, MMP13, and ADAMTS5 protein levels in chondrocytes under different co-culture conditions. n = 3. J . Flow cytometry analysis of chondrocyte proliferation under different co-culture conditions, with quantitative analysis. n = 6. K . Representative EdU staining images and quantitative analysis of chondrocyte proliferation. Scale bar = 100 μm. n = 6. Data are presented as mean ± SD. One-way ANOVA was used for multiple group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: RNA Sequencing, Control, Activity Assay, Western Blot, Knockdown, Expressing, Quantitative RT-PCR, Staining, Co-Culture Assay, Flow Cytometry

Journal: Journal of Translational Medicine

Article Title: Glibenclamide attenuates osteoarthritis by suppressing NLRP3 inflammasome activation in synovial macrophages through the MAPK pathway

doi: 10.1186/s12967-026-07923-7

Figure Lengend Snippet: In vivo knockdown of synovial macrophage NLRP3 weakens Gb-mediated protection against OA. A . Representative IF images of ZsGreen distribution in the knee joint after intra-articular injection of rAAV-shNLRP3, with quantitative analysis of ZsGreen-positive cells in synovium and cartilage. S indicates synovium, and C indicates cartilage. Scale bar = 1 mm (left), 50 μm (right). n = 3. B . Representative IHC images of NLRP3 in synovium and IF images of F4/80 and NLRP3 co-staining in synovium. Scale bar = 100 μm. n = 6. C . Representative HE, SO-FG, and TB staining of synovium and cartilage. Scale bar = 100 μm. n = 6. D . Synovitis score comparing severity of synovial inflammation. n = 6. E. OARSI score comparing cartilage degradation. n = 6. F . Representative IHC images of COL2A and MMP13 in cartilage. Scale bar = 100 μm. n = 6. G . Quantitative analysis of COL2A and MMP13 positive areas. n = 6. H . Representative micro-CT 3D reconstruction images showing osteophytes (red arrows) and quantitative analysis of osteophyte numbers. n = 6. I. Representative coronal micro-CT images, with quantitative analysis of subchondral BV/TV and Tb.Th. n = 6. Data are presented as mean ± SD. Student’s t-test was used for two-group comparisons, and one-way ANOVA for multiple-group comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: MMP13 , Proteintech , 18165-1-AP.

Techniques: In Vivo, Knockdown, Injection, Staining, Micro-CT